Actin gels
نویسندگان
چکیده
Solutions and gels of the filamentous protein F-actin have not only been the subject of numerous recent biophysical studies, but have also proven to be quite fruitful model systems for fundamental polymer science. They are particularly good models of ‘semiflexible’ polymers, because they have an enormous ratio of persistence length to molecular diameter with characteristic mesh sizes of the order of one micron, which has permitted both a direct optical visualization of these networks and a number of recent experimental techniques for local viscoelastic characterization.
منابع مشابه
Detection of actin-binding proteins in human platelets by 125I-actin overlay of polyacrylamide gels
Actin-binding proteins have been identified in human platelets with a gel-overlay technique that uses 125I-G-actin. Platelet proteins were separated on SDS polyacrylamide gels using the buffer system of Laemmli (1970, Nature [Lond.] 227:680-685). The proteins were fixed in the gels with methanol-acetic acid, the SDS was washed out, and the proteins were renatured. The gels were incubated with 1...
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